【英文标准名称】:StandardGuideforConductingLaboratorySoilToxicityorBioaccumulationTestswiththeLumbricidEarthwormEiseniaFetidaandtheEnchytraeidPotwormEnchytraeusalbidus
【原文标准名称】:用蚯蚓(LumbricidEarthwormEiseniaFetida和EnchytraeidPotwormEnchytraeusalbidus)做实验室土壤毒性试验或生物累积试验的标准指南
【标准号】:ASTME1676-2004
【标准状态】:现行
【国别】:
【发布日期】:2004
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:E47.02
【标准类型】:(Guide)
【标准水平】:()
【中文主题词】:毒性;实验室;底部;毒理学;土壤;污染控制
【英文主题词】:bioaccumulationtests;earthworm;potworms;soiltoxicity
【摘要】:1.1Thisguidecoversproceduresforobtaininglaboratorydatatoevaluatetheadverseeffectsofcontaminants(forexample,chemicalsorbiomolecules)associatedwithsoiltoearthworms(FamilyLumbricidae)andpotworms(FamilyEnchytraeidae)fromsoiltoxicityorbioaccumulationtests.Themethodsaredesignedtoassesslethalorsublethaltoxiceffectsonearthwormsorbioaccumulationofcontaminantsinshort-termtests(7to28days)oronpotwormsinshorttolong-termtests(14to42days)interrestrialsystems.Soilstobetestedmaybe(1)referencesoilsorpotentiallytoxicsitesoils;(2)artificial,reference,orsitesoilsspikedwithcompounds;(3)sitesoilsdilutedwithreferencesoils;or(4)siteorreferencesoilsdilutedwithartificialsoil.TestproceduresaredescribedforthespeciesEiseniafetida(see)andforthespeciesEnchytraeusalbidus(see).Methodsdescribedinthisguidemayalsobeusefulforconductingsoiltoxicitytestswithotherlumbricidandenchytraeidterrestrialspecies,althoughmodificationsmaybenecessary.1.2Modificationoftheseproceduresmightbejustifiedbyspecialneeds.Theresultsoftestsconductedusingatypicalproceduresmaynotbecomparabletoresultsusingthisguide.Comparisonofresultsobtainedusingmodifiedandunmodifiedversionsoftheseproceduresmightprovideusefulinformationconcerningnewconceptsandproceduresforconductingsoiltoxicityandbioaccumulationtestswithterrestrialworms.1.3Theresultsfromfield-collectedsoilsusedintoxicityteststodetermineaspatialortemporaldistributionofsoiltoxicitymaybereportedintermsofthebiologicaleffectsonsurvivalorsublethalendpoints(seeSection).Theseprocedurescanbeusedwithappropriatemodificationstoconductsoiltoxicitytestswhenfactorssuchastemperature,pH,andsoilcharacteristics(forexample,particlesize,organicmattercontent,andclaycontent)areofinterestorwhenthereisaneedtotestsuchmaterialsassewagesludgeandoils.Thesemethodsmightalsobeusefulforconductingbioaccumulationtests.1.4Theresultsoftoxicitytestswith(1)materials(forexample,chemicalsorwastemixtures)addedexperimentallytoartificialsoil,referencesoils,orsitesoils,(2)sitesoilsdilutedwithreferencesoils,and(3)siteorreferencesoilsdilutedwithartificialsoil,soastocreateaseriesofconcentrations,maybereportedintermsofanLC50(medianlethalconcentration)andsometimesanEC50(medianeffectconcentration).TestresultsmaybereportedintermsofNOEC(noobservedeffectconcentration),LOEC(lowestobservedeffectconcentration)orasanECx(concentrationwherex%reductionofabiologicaleffectoccurs.BioaccumulationtestresultsarereportedasthemagnitudeofcontaminantconcentrationaboveeithertheDay0tissuebaselineanalysisortheDay28tissuesfromthenegativecontrolorreferencesoil(thatis,2,5,10)(see).1.5Thisguideisarrangedasfollows:ScopeReferencedDocumentsTerminologySummaryofGuideSignificanceandUseInterferencesApparatusSafetyPrecautionsSoilTestOrganismProcedureAnalyticalMethodologyAcceptabilityofTestCalculationofResultsReportAnnexes.Eiseniafetida.ArtificialSoilComposition.BioaccumulationTestingUtilizingEiseniafetida.Enchytra......
【中国标准分类号】:Z18
【国际标准分类号】:13_020_40;13_080_30
【页数】:26P.;A4
【正文语种】: